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Variability in plants regenerated from tissue culture

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Published by Praeger in New York, N.Y .
Written in English


  • Regeneration (Botany),
  • Plant cell culture.,
  • Plant tissue culture.

Book details:

Edition Notes

Includes bibliographies and index.

Statementedited by Elizabeth D. Earle, Yves Demarly.
ContributionsEarle, Elizabeth D., Demarly, Yves.
LC ClassificationsQK840 .V37 1982
The Physical Object
Paginationxvii, 392 p. :
Number of Pages392
ID Numbers
Open LibraryOL3485851M
ISBN 100030593646
LC Control Number82005397

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  Although somaclonal variation has been studied extensively, the mechanisms by which it occurs remain largely either unknown or at the level of theoretical speculation in perennial fruit crops [14,15].A variety of factors may contribute to the system by which the regeneration is induced, type of tissue, explant source, media components and the duration of the culture cycle are Cited by: Variability was observed among the wheat genotypes tested for callus induction, regenerable callus formation, response to subculture, and plant regeneration potential. Eighteen genotypes were capable of regenerating plants after four subcultures (90 to days). Cultures from five genotypes remained totipotent after by: In book: Recent advances in plant in vitro culture, Chapter: Somaclonal Variation in Tissue Culture: A Case Study with Olive, Publisher: Intech, Editors: Annarita Leva and Laura M.R. Rinaldi, pp.   This raised the question of how tissue culture processes affect the epigenome of regenerated plants derived from tissue culture. Changes in the epigenome have been proposed to be a source of somaclonal variation (i.e., phenotypic variation among regenerated plants) for decades (Kaeppler and Phillips, ; Kaeppler et al., ; Thorpe,

However, plant tissue culture is known to engender frequent unwanted variation, termed somaclonal variation. To evaluate the contribution of large-scale genome instability to this phenomenon, we analyzed potatoes (Solanum tuberosum) regenerated from either protoplasts or stem explants for copy number changes by comparison of Illumina read depth. Qualitative variation was noted in self‐pollinated progeny of plants regenerated from culture of two inbreds. Chimerism in regenerated plants was indicated by sectoring of the genetic markers. Some culture‐induced variant phenotypes were similar to known spontaneous mutation in sunflower but others have been not yet described. Abstract. Somaclonal variation (SC) in plants regenerated from tissue culture, via organogenesis or somatic embryogenesis, is frequently associated with abnormalities in the content of deoxyribonucleic acid (DNA), viz., aneuploidy and polyploidy. Tissue culture is an essential tool for the regeneration of uniform plant material. However, tissue culture conditions can be a source of abiotic stress for plants, leading to changes in the DNA sequence and methylation patterns. Despite the growing evidence on biochemical processes affected by abiotic stresses, how these altered biochemical processes affect DNA sequence and methylation.

A plant breeder may use tissue culture to screen cells rather than plants for advantageous characters, e.g. herbicide resistance/tolerance. Large-scale growth of plant cells in liquid culture. Recent advances in maize(Zea mays L.) tissue culture methods permit reproducible establishment of rapid‐growing, friable, embryogenic (Type‐II) cultures from some gh Type‐II cultures have replaced the more common Type‐I (relatively compact, organogenic) cultures for many applications, little information is available concerning somaclonal variation in plants regenerated. Plant Tissue Culture Terminology AdventitiousDeveloping from unusual points of origin, such as shoot or root tissues, from callus or embryos, from sources other than zygotes. Agara polysaccharide powder derived from algae used to gel a medium. Agar is generally used at a concentration of g/liter. This symposium is the third in a series featuring the propaga­ tion of higher plants through tissue culture. The first of these symposia, entitled "A Bridge Between Research and Application," was held at the University in and was published by the Technical Information Center, Department of Energy.